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Flow Cytometry Immunophenotyping

Important Note

Immunophenotypes are a collection of related flow cytometric assays used to aid in the identification and characterization of neoplastic disorders of the hematopoetic system. Testing can be performed on blood, bone marrow aspirates, non-GYN fluids, and tissue biopsies. The testing is high complexity, expensive, and time sensitive. Consultation with Hematology/Oncology staff and/or the on-call hematopathologist is strongly advised.

eDH Procedure Code(s)

LAB1998 Immunophenotyping Flow Cytometry (Non-blood)
LAB3786 Immunophenotyping Flow Cytometry (Blood)
LAB1834 Immunophenotyping Flow Cytometry (Blood/Bone Marrow) —LAB ONLY
LAB2302 Immunophenotyping Flow Cytometry (Tissue) —LAB ONLY
LAB2901 CTCL

Order Questions

LAB1998 Immunophenotyping Flow Cytometry (Non-blood)
  • Indication for study:
LAB3786 Immunophenotyping Flow Cytometry (Blood)
  • Panel requested:
  • Is patient on Rituximab, Daratumumab, Other?
  • Indication for study:
LAB2302 Immunophenotyping Flow Cytometry (Tissue) --LAB ONLY
  • Specimen Type:
  • Specimen Source:
  • Indication for study:
LAB2901 CTCL
  • Is this the first time this patient is being evaluated by flow cytometry for CTCL?

Collection Information

Specimen Type Container Minimum Volume Special Handling
Blood Lav-R 3 mL Do not spin
Body Fluid
Brushing
CSF
Washing		 SterCont-R

Separate from other collection container		 1 mL Do not spin
FNA SterCont-R with 5 or 10 mL of RPMI or saline

Separate from other collection container		 As much as possible DO NOT ADD FIXATIVE
Tissue SterCont-R with 5 or 10 mL of RPMI or saline 5 mm3 of fresh tissue DO NOT ADD FIXATIVE

Specimen Information

Specimen Type Specimen Source Acceptable Containers Minimum Test Volume (mL)
Blood Blood, Venous Lav4-A 1.0
Blood Blood, Venous Lav6-A 1.0
Blood Blood, Venous LavM-R 1.0
Blood Blood, Venous Lav4-R 1.0
Blood Blood, Venous Lav6-R 1.0
Bone Marrow Varies LavBM-A 1.0
Varies Varies SterCont-R 1.0
Cerebrospinal Fluid Cerebrospinal Fluid CSF-R 1.0

Shipping and Handling

Processing Instructions:

Blood: DO NOT SPIN

Body Fluid, Brushing, CSF, Washing, FNA, Tissue: DO NOT ADD FIXATIVE

 

Transport Instructions:

Blood: Ambient

Body Fluid, Brushing, CSF, Washing, FNA, Tissue: Refrigerated

 

Specimen Stability: 48 hours

 

Test Components

Component LOINC Code
CD14/FLAER- % MONOCYTE FLOW 90736-0
CD24/FLAER- % GRANULOCYTE FLOW 90737-8
CD3+ % FLOW 20599-7
CD3+ ABSOLUTE FLOW 20598-9
CD3/CD4+ % FLOW 32516-7
CD3/CD4+ ABSOLUTE FLOW 32515-9
CD3/CD4+ CD26 - % FLOW 55230-7
CD3/CD4+ CD26- ABSOLUTE FLOW 55230-7
CD3/CD4+ CD7- % FLOW 55230-7
CD3/CD4+ CD7- ABSOLUTE FLOW 55230-7
CD3/CD4+ CD7/CD26- % FLOW 55230-7
CD3/CD4+ CD7/CD26- ABSOLUTE 55230-7
CD3/CD8+ % FLOW 55230-7
CD38 BRIGHT/CD138+ FLOW 55230-7
CD45 DIM % FLOW 20610-2
CD4:CD8 RATIO FLOW 54218-3
CELL VIABILITY FLOW 46696-1
CLINICAL INFORMATION FLOW 62364-5
DIAGNOSIS COMMENT (AP) 73703-1
DISCLAIMER FLOW 62364-5
FINAL DIAGNOSIS (AP) 34574-4
GATING B-CELL:T-CELL RATIO FLOW 46696-1
GATING CD19+ % FLOW 46696-1
GATING CD3+ % FLOW 46696-1
GATING CD56+ % FLOW 46696-1
GRANULOCYTE % FLOW 61124-4
INTERPRETATION 78210-2
KAPPA:LAMBDA RATIO FLOW 21375-1
LYMPHOCYTE % FLOW 61123-6
MONOCYTE % FLOW 61125-1
SPECIMEN PROCESSING FLOW 46696-1
TYPE I RBCS % FLOW 17177-7
TYPE II RBCS % FLOW 33663-6
TYPE III RBCS % FLOW 90738-6

Turnaround Time (TAT)

72 Hours

CPT Code(s)

88184

Performing Lab Section

Flow Cytometry

Performing Lab(s)

Dartmouth Hitchcock Medical Center Laboratory (MHMH)