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HelpLower Respiratory Culture
eDH Procedure Code(s)
LAB267
Test Information
A gram stain is performed to evaluate specimen quality. Sputum specimens with >25 squamous epithelial cells will not be cultured. Tracheal aspirates with no microorganisms seen or >10 squamous epithelial cells will not be cultured.
Collection Information
If Mycobacterial (AFB) or Fungal testing is also requested send as much specimen as possible for optimal recovery of organisms.
Expectorated sputum: If possible, have the patient rinse mouth and gargle with water prior to sputum collection. Instruct the patient not to expectorate saliva or postnasal discharge into the container. Collect specimen resulting from deep cough in sterile screw-cap cup or other suitable sterile collection assembly.
Induced sputum (collected by respiratory therapy): Using a wet toothbrush, brush the bucal mucosa, tongue, and gums prior to the procedure. Rinse the patient`s mouth thoroughly with water. Using an ultrasonic nebulizer, have the patient inhale approximately 20-30 mL of 3-10% 0.85% NaCl. Collect the induced sputum in a sterile screw-cap cup or other suitable sterile collection assembly.
Tracheal aspirations: Aspirate the specimen into a sterile sputum trap.
Specimen Information
| Specimen Type | Specimen Source | Acceptable Containers | Minimum Test Volume (mL) | |
|---|---|---|---|---|
| Varies | Varies | SterCup-R | 1.0 | |
Shipping and Handling
Transport Temperature: Refrigerate
Specimen Stability
Ambient: n/a
Refrigerated: 24 hours
Frozen: n/a
Test Components
| Component | LOINC Code |
|---|---|
| GRAM STAIN | 664-3 |
| LOWER RESPIRATORY CULTURE | 32355-0 |
Turnaround Time (TAT)
2 Days
CPT Code(s)
87070
Performing Lab Section
Microbiology
Performing Lab(s)
Cheshire Medical Center Laboratory (CHE)
Dartmouth Hitchcock Medical Center Laboratory (MHMH)