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Methotrexate

eDH Procedure Code(s)

LAB481 Methotrexate Level

Collection Information

Samples drawn for methotrexate therapeutic drug monitoring are ideally drawn via peripheral venipuncture. When peripheral venipuncture is not possible refer to DH procedure ID 234 for guideance on appropriate collection site and steps to adequately flush the catheter/line. Subcutaneous ports (e.g. Mediports) should be avoided due to high risk of sample contamination.
https://dh.navexone.com/content/dotNet/documents/?docid=55170

Specimen Information

Specimen Type Specimen Source Acceptable Containers Minimum Test Volume (mL)
Blood Blood, Venous Gold-R 1.0

Shipping and Handling

Processing Instructions: N/A

Transport Temperature: Refrigerated
Transport Instructions: N/A

Specimen Stability Unprocessed
Unprocessed: 2 Hours
Specimen Stability Post Processing
Ambient: 3 Days
Refrigerate: 2 Weeks
Frozen: 2 Weeks

Test Components

Component LOINC Code
METHOTREXATE 14836-1

Turnaround Time (TAT)

STAT 1 Hour
Routine 4 Hours

CPT Code(s)

LAB481 Methotrexate level: 80204

Performing Lab Section

Chemistry

Performing Lab(s)

Dartmouth Hitchcock Medical Center Laboratory (MHMH)