Additional Testing Information

This assay assesses the exonic regions of genes which have been described in the medical literature relevant in solid tumors.  The following variant types may be detected: Single nucleotides variants, insertions, deletions, gene copy number gains, and fusions.  In addition, tumor mutation burden (TMB) and Microsatellite instability (MSI) status is reported. 
 

CGAT – DHCancerSeq Gene list v2 (4/8/2025)

The following genes are tested for single nucleotide variants, small insertions,  and small deletions(204):
ABRAXAS1, ACVR1, AKT1, AKT2, AKT3, ALK, APC, AR, ARID1A, ARID1B, ARID2, ATM, ATR, ATRX, BAP1, BARD1, BCL2, BCL6, BRAF, BRCA1, BRCA2, BRIP1, BTK, CARD11, CBL, CCND1, CCND2, CCND3, CCNE1, CD274, CD79A, CD79B, CDH1, CDK12, CDK4, CDK6, CDKN1B, CDKN2A, CDKN2B, CDKN2C, CEBPA, CHEK1, CHEK2, CREBBP, CSF1R, CSF3R, CTNNB1, DAXX, DDR2, DICER1, DNMT3A, EGFR, EP300, EPCAM, ERBB2, ERBB3, ERBB4, ERCC1, ERCC2, ERG, ESR1, ETV6, EZH2, FANCA, FANCI, FANCL, FAT1, FBXW7, FGF1, FGF10, FGF14, FGF19, FGF2, FGF23, FGF3, FGF4, FGF5, FGF6, FGF7, FGF8, FGF9, FGFR1, FGFR2, FGFR3, FGFR4, FH, FLT1, FLT3, FOXL2, GATA1, GATA2, GATA3, GEN1, GNA11, GNAQ, GNAS, H3-3A, HNF1A, HRAS, IDH1, IDH2, INPP4B, JAK2, JAK3, KDR, KEAP1, KIT, KMT2A, KMT2C, KRAS, LAMP1, MAP2K1, MAP2K2, MAP3K1, MAP3K13, MAPK, MAPK1, MCL1, MDM2, MDM4, MED12, MET, MLH1, MLLT3, MPL, MRE11, MSH2, MSH3, MSH6, MTOR, MUTYH, MYC, MYCL, MYCN, MYD88, NBN, NF1, NF2, NFE2L2, NOTCH1, NOTCH2, NOTCH3, NPM1, NRAS, NRG1, PALB2, PBRM1, PDGFRA, PDGFRB, PIK3CA, PIK3CB, PIK3CD, PIK3CG, PIK3R1, PIM1, PMS2, POLD1, POLE, PPM1D, PPP2R2A, PTCH1, PTEN, PTPN11, PTPRD, PTPRT, RAD51, RAD51B, RAD51C, RAD51D, RAD54L, RAF1, RB1, RET, RICTOR, ROS1, RPS6KB1, RUNX1, SDHB, SDHD, SETBP1, SETD2, SLX4, SMAD4, SMARCA4, SMARCB1, SMO, SRC, STK11, SUFU, TERT, TET2, TFRC, TP53, TRAF7, TSC1, TSC2, VHL, WRN, WT1, XPC, XPO1, XRCC2, YAP1, ZFHX3

The following genes are tested for amplifications (12):
CCND1, CCNE1, CDK12, CDK4, EGFR, ERBB2, FGFR1, MDM2, MET, MYB, MYC, MYCN

Biomarkers (2):
TMB, MSI

The following genes are tested for fusions (778):
ABI1, ABL1, ABL2, ACACA, ACE, ACER1, ACKR3, ACSL3, ACSL6, ACTB, ACTL6A, ADD3, ADGRG7, AFAP1, AFDN, AFF1, AFF3, AFF4, AGBL1, AGBL4, AGGF1, AGK, AGR3, AHI1, AHRR, AKAP13, AKAP9, AKT1, AKT3, ALK, ANKRD28, APC, AR, ARHGAP20, ARHGAP26, ARHGEF12, ARID1A, ARNT, ASPSCR1, ASTN2, ASXL1, ATF1, ATG5, ATG7, ATIC, ATP1B4, AUTS2, AXL, BACH2, BAG4, BAIAP2L1, BANP, BAZ2A, BCAN, BCAS3, BCAS4, BCL10, BCL11A, BCL11B, BCL2, BCL2L1, BCL2L11, BCL3, BCL6, BCL7A, BCL9, BCOR, BCORL1, BCR, BDNF, BEND2, BICC1, BIRC3, BIRC6, BRAF, BRCA1, BRCA2, BRD1, BRD3, BRD4, BRD8, BRWD3, BTBD1, BTBD18, BTG1, C2CD2L, C8orf34, CALCA, CAMTA1, CANT1, CAPRIN1, CAPZA2, CARMN, CARS1, CASP7, CBFA2T3, CBFB, CBL, CCAR2, CCDC149, CCDC170, CCDC28A, CCDC30, CCDC6, CCDC88C, CCNB1IP1, CCNB3, CCND1, CCND2, CCND3, CCNY, CD274, CD63, CD74, CDH11, CDK4, CDK5RAP2, CDK6, CDKN2A, CDKN2D, CDX1, CDX2, CEBPA, CEBPB, CEBPD, CEBPE, CEP128, CEP170B, CEP43, CEP85L, CFAP68, CHCHD7, CHD6, CHEK2, CHGA, CHIC2, CHMP2B, CHN1, CHST11, CIC, CIITA, CIP2A, CITED1, CITED2, CLCN6, CLIP2, CLP1, CLTC, CLTCL1, CMKLR1, CNBP, CNOT2, CNTRL, COA5, COG5, COL1A1, COL1A2, COL3A1, COL6A3, COX6C, CPSF6, CRADD, CREB1, CREB3L1, CREB3L2, CREBBP, CREM, CRLF2, CRTC1, CRTC3, CSF1, CSF1R, CTDSP2, CTLA4, CTNNB1, CTNNBL1, CUX1, CXXC5, DAB2IP, DACH1, DACH2, DDIT3, DDX10, DDX20, DDX31, DDX3X, DDX5, DDX6, DEK, DHH, DICER1, DIP2C, DMRT1, DNAJB1, DPM1, DUSP22, DUX4, DUX4L1, DVL2, EBF1, EEFSEC, EGFR, EGR1, EGR2, EGR3, EGR4, EIF1AX, EIF4A2, ELAVL3, ELF4, ELK4, ELL, ELN, EML1, EML4, EP300, EP400, EPC1, EPC2, EPOR, EPS15, ERBB2, ERBB3, ERBB4, ERC1, ERCC1, ERG, ERLIN2, ESR1, ESRP1, ETS1, ETV1, ETV4, ETV5, ETV6, EWSR1, EZH1, EZHIP, EZR, FAM114A2, FAM118B, FAM131B, FAM193A, FARSB, FCGR2B, FCRL4, FEN1, FEV, FGF8, FGFR1, FGFR1OP2, FGFR2, FGFR3, FGFR4, FHIT, FIP1L1, FKBP15, FLI1, FLNA, FLT3, FLT3LG, FN1, FNBP1, FOSB, FOSL1, FOXO1, FOXO3, FOXO4, FOXP1, FOXR2, FRK, FRYL, FSTL3, FUS, FXR1, FYCO1, GAS5, GAS7, GATA1, GFI1, GFI1B, GFPT1, GIT2, GLI1, GLI2, GLIS1, GLIS2, GLIS3, GMPS, GNAI1, GNAS, GOLGA5, GOPC, GORASP2, GOSR1, GOT1, GPHN, GPR34, GREB1, GRHPR, GRID1, GRM1, GTF2I, GTF2IRD1, H2AX, H4C13, H4C9, HAS2, HERPUD1, HEY1, HHEX, HIP1, HIPK1, HLF, HMGA1, HMGA2, HMGN2P46, HNF1A, HNRNPA2B1, HOOK3, HOXA10, HOXA11, HOXA13, HOXA3, HOXA9, HOXC11, HOXC13, HOXD11, HOXD13, HRAS, HSP90AA1, HSP90AB1, ID4, IDH1, IDH2, IGF2BP3, IKZF1, IL2, IL21R, IL2RB, IL3, INPP5D, IQCG, IRAG2, IRF2BP2, IRF4, IRS4, ITK, JAK1, JAK2, JAK3, JAZF1, JPX, KANK1, KAT6A, KAT6B, KDM2A, KDM2B, KDM5A, KDR, KDSR, KIAA1217, KIAA1549, KIF5B, KIRREL1, KIT, KLF17, KLK1, KLK2, KMT2A, KNL1, KPNB1, KRAS, KRT20, KRT7, KSR1, KTN1, LAMTOR1, LASP1, LCK, LCP1, LGR5, LHFPL6, LHX2, LHX4, LIFR, LMBRD1, LMNA, LMO1, LMO2, LNP1, LPP, LPXN, LRRC37B, LTBP1, LTK, LYL1, MACF1, MACROD1, MAF, MAFB, MALT1, MAML1, MAML2, MAML3, MAMLD1, MAP2K1, MAP3K8, MAPRE1, MAST1, MAST2, MBNL1, MBTD1, MDS2, MEAF6, MECOM, MED12, MEIS1, MEN1, MERTK, MET, MITF, MKRN1, MLF1, MLLT1, MLLT10, MLLT11, MLLT3, MLLT6, MMP16, MN1, MNX1, MPRIP, MRTFA, MRTFB, MSH2, MSI2, MSN, MUC1, MUTYH, MYB, MYBL1, MYC, MYH11, MYH9, MYLK, MYO18A, MYO1F, NAB2, NACA, NACC2, NAPA, NAV1, NBEAP1, NBR1, NCOA1, NCOA2, NCOA3, NCOA4, NDE1, NDRG1, NF1, NF2, NFASC, NFATC1, NFATC2, NFIB, NFKB2, NGF, NGFR, NIN, NIPBL, NKX2-5, NONO, NOTCH1, NOTCH2, NPM1, NR1D1, NR4A3, NR6A1, NRAS, NRF1, NRG1, NSD1, NSD2, NSD3, NT5C2, NTF3, NTF4, NTRK1, NTRK2, NTRK3, NUMA1, NUP107, NUP214, NUP98, NUTM1, NUTM2A, NUTM2B, OFD1, OGA, OGT, OLIG2, OLR1, OMD, OPHN1, P2RY8, PAFAH1B2, PAN3, PAPPA, PATZ1, PAX3, PAX5, PAX7, PAX8, PBX1, PBX3, PCDHGA1, PCM1, PCSK5, PCSK7, PDCD1LG2, PDE4DIP, PDGFB, PDGFRA, PDGFRB, PDPN, PER1, PGK1, PGR, PHF1, PHF23, PICALM, PIK3CA, PIM1, PKD1, PLAG1, PLPP3, PML, POM121, POR, POU2AF1, POU2AF3, POU5F1, PPARG, PPARGC1A, PPFIBP1, PPP1CB, PPP2R1B, PRCC, PRDM1, PRDM10, PRDM16, PRKACA, PRKAR1A, PRKCA, PRKCB, PRKCD, PRKCG, PRKD1, PRKD2, PRKD3, PRKDC, PRKG2, PRRX1, PRRX2, PSIP1, PSMD2, PTBP1, PTCH1, PTEN, PTH, PTK7, PTPRK, PTPRR, PTPRZ1, PVT1, QKI, RAB2A, RABEP1, RAD51B, RAF1, RALGDS, RANBP2, RAP1GDS1, RARA, RASGEF1A, RBM15, RBM6, RBPMS, RCOR1, RCSD1, RECK, RELA, RET, RHEBL1, RHOH, RNF130, RNF213, ROS1, RPL22, RPN1, RPS6KC1, RRAGB, RREB1, RRM1, RSPO3, RTEL1, RUNX1, RUNX1T1, RUNX2, S100A10, SARNP, SATB2, SDC4, SEC31A, SEPTIN14, SEPTIN2, SEPTIN5, SEPTIN6, SEPTIN9, SERPINE1, SERPINF1, SET, SETBP1, SFMBT1, SFPQ, SH3D19, SH3GL1, SHTN1, SIK3, SLC1A2, SLC26A11, SLC34A2, SLC44A1, SLC45A3, SLC5A5, SLCO1B3, SLIT1, SMAP1, SMARCA5, SMARCB1, SND1, SNHG5, SNURF, SNX29, SORBS2, SORT1, SP3, SPECC1, SPECC1L, SPTBN1, SQSTM1, SRF, SRGAP3, SRSF3, SS18, SS18L1, SSBP2, SSX1, SSX2, SSX4, SSX4B, ST6GAL1, STAT5B, STAT6, STIL, STK11, STRN, SUGCT, SUGP2, SUZ12, SYK, SYN2, TACC1, TACC2, TACC3, TAF15, TAFA2, TAL1, TAL2, TAOK1, TBL1XR1, TBX15, TCEA1, TCF12, TCF7L1, TCF7L2, TCL1A, TCTA, TEAD1, TEAD2, TEAD3, TEAD4, TEC, TENM1, TERT, TET1, TFCP2, TFE3, TFG, TFPT, TFRC, TGFBR3, THADA, THRAP3, TIRAP, TLX1, TLX3, TMPRSS2, TNFRSF11A, TNFRSF17, TOP1, TOP2B, TP53, TP53BP1, TP63, TPM3, TPM4, TPR, TRA2A, TRHDE, TRIM11, TRIM24, TRIM27, TRIM33, TRIM61, TRIP11, TRPS1, TSC2, TSHR, TTL, TYK2, UACA, UBE2J2, UBTF, USP16, USP42, USP6, VCL, VGLL2, VGLL3, VHL, VIM, VTI1A, WARS1, WASF2, WDFY2, WDR18, WDR70, WIF1, WSB1, WT1, WWTR1, XIAP, YAP1, YPEL5, YTHDF2, YWHAE, ZBTB16, ZBTB8A, ZC3H7A, ZC3H7B, ZC3HAV1, ZFP36, ZFP64, ZFPM2, ZFTA, ZFYVE19, ZMIZ1, ZMYM2, ZMYND11, ZNF207, ZNF384, ZNF444, ZNF521, ZNF585B, ZNF687

The following Splice variants are reported (3):
MET exon 14 skipping variants, EGFRvIII, ARv7,