Additional Testing Information

This assay assesses the exonic regions of genes which have been described in the medical literature relevant in solid tumors.  The following variant types may be detected: Single nucleotides variants, insertions, deletions, gene copy number gains, and fusions.  In addition, tumor mutation burden (TMB) status is reported. 

CGAT – DHCancerSeq Gene list v1.1 (9/11/2023)

The following genes are tested for single nucleotide variants, small insertions, deletions and amplifications (204):
ABRAXAS1, ACVR1, AKT1, AKT2, AKT3, ALK, APC, AR, ARID1A, ARID1B, ARID2, ATM, ATR, ATRX, BAP1, BARD1, BCL2, BCL6, BRAF, BRCA1, BRCA2, BRIP1, BTK, CARD11, CBL, CCND1, CCND2, CCND3, CCNE1, CD274, CD79A, CD79B, CDH1, CDK12, CDK4, CDK6, CDKN1B, CDKN2A, CDKN2B, CDKN2C, CEBPA, CHEK1, CHEK2, CREBBP, CSF1R, CSF3R, CTNNB1, DAXX, DDR2, DICER1, DNMT3A, EGFR, EP300, EPCAM, ERBB2, ERBB3, ERBB4, ERCC1, ERCC2, ERG, ESR1, ETV6, EZH2, FANCA, FANCI, FANCL, FAT1, FBXW7, FGF1, FGF10, FGF14, FGF19, FGF2, FGF23, FGF3, FGF4, FGF5, FGF6, FGF7, FGF8, FGF9, FGFR1, FGFR2, FGFR3, FGFR4, FH, FLT1, FLT3, FOXL2, GATA1, GATA2, GATA3, GEN1, GNA11, GNAQ, GNAS, H3-3A, HNF1A, HRAS, IDH1, IDH2, INPP4B, JAK2, JAK3, KDR, KEAP1, KIT, KMT2A, KMT2C, KRAS, LAMP1, MAP2K1, MAP2K2, MAP3K1, MAP3K13, MAPK, MAPK1, MCL1, MDM2, MDM4, MED12, MET, MLH1, MLLT3, MPL, MRE11, MSH2, MSH3, MSH6, MTOR, MUTYH, MYC, MYCL, MYCN, MYD88, NBN, NF1, NF2, NFE2L2, NOTCH1, NOTCH2, NOTCH3, NPM1, NRAS, NRG1, PALB2, PBRM1, PDGFRA, PDGFRB, PIK3CA, PIK3CB, PIK3CD, PIK3CG, PIK3R1, PIM1, PMS2, POLD1, POLE, PPM1D, PPP2R2A, PTCH1, PTEN, PTPN11, PTPRD, PTPRT, RAD51, RAD51B, RAD51C, RAD51D, RAD54L, RAF1, RB1, RET, RICTOR, ROS1, RPS6KB1, RUNX1, SDHB, SDHD, SETBP1, SETD2, SLX4, SMAD4, SMARCA4, SMARCB1, SMO, SRC, STK11, SUFU, TERT, TET2, TFRC, TP53, TRAF7, TSC1, TSC2, VHL, WRN, WT1, XPC, XPO1, XRCC2, YAP1, ZFHX3

The following genes are tested for fusions (55):
ABL1, AKT3, ALK, AR, AXL, BCL2, BRAF, BRCA1, BRCA2, CDK4, CSF1R, EGFR, EML4, ERBB2, ERG, ESR1, ETS1, ETV1, ETV4,ETV5, EWSR1, FGFR1, FGFR2, FGFR3, FGFR4, FLI1, FLT1, FLT3, JAK2, KDR, KIF5B, KIT, KMT2A, MET, MLLT3, MSH2, MYC, NOTCH1, NOTCH2, NOTCH3, NRG1, NTRK1, NTRK2, NTRK3, PAX3, PAX7, PDGFRA, PDGFRB, PIK3CA, PPARG, RAF1, RET, ROS1, RPS6KB1, TMPRSS2

The following Splice variants are reported (3):
EGFRvIII, ARv7, MET exon 14 skipping variants.

Biomarkers (1):
TMB, MSI