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Activated Protein C (APC) Resistance

eD-H Order Code

LAB846

Specimen Collection Information

Specimen Minimum Test Volume Container Special Handling
Blood 3.0 ML  

Specimen Transport Temperature

Double spin, separate, and freeze plasma
Freeze red cells in separate tube
Lavender top: send whole blood, Refrigerated.

Specimen Stability

4 hours

Additional Specimen Instructions

1.8 mL Sodium Citrate tube filled to the line

OR

2.7 mL Sodium Citrate line filled to the line

AND

2.0 mL EDTA tube

 

If low APC Resistance (<2.54) is found, the Lavender tube is sent to Molecular Genetics for confirmation by the Factor V DNA mutation test.

 

Blue top tubes must be filled within 10% of stated volume. A discard tube must be used if a citrate tube is to be drawn using a winged blood collection set. It is important to remove air from the blood collection set to ensure the proper blood volume is obtained in the coag tube.

 

If the patient has a known hematocrit >55%, please contact the coagulation lab for an adjusted collection tube before drawing the blood.

Reference Ranges / Critical Values

Assay Gender Age Range Normal Range Critical Values Units
APC All 0 Minutes – 150 Years >2.54    

Performing Lab Section

Hematology

CPT(s)

85307