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HelpAPC Resistance
eD-H Order Code
LAB846
Specimen Collection Information
| Specimen | Minimum Test Volume | Container | Special Handling |
|---|---|---|---|
| Blood |
PEDI, PICU, or ICN draws refer to specimen collection job aid. All other Coag tubes must be filled to the line on tube. |
Specimen Transport Temperature
Double spin, separate, and freeze plasma
Freeze red cells in separate tube
Lavender top: send whole blood, Refrigerated.
Reference Ranges / Critical Values
| Assay | Gender | Age Range | Normal Range | Critical Values | Units |
|---|---|---|---|---|---|
| APC | All | 0 Minutes – 150 Years | >2.40 |
Specimen Stability
4 hours
Additional Specimen Instructions
1.8 mL Sodium Citrate tube filled to the line
OR
2.7 mL Sodium Citrate tube filled to the line
AND
2.0 mL EDTA tube
If low APC Resistance (<2.40) is found, the Lavender tube is sent to Molecular Genetics for confirmation by the Factor V DNA mutation test.
Blue top tubes must be filled within 10% of stated volume. A discard tube must be used if a citrate tube is to be drawn using a winged blood collection set. It is important to remove air from the blood collection set to ensure the proper blood volume is obtained in the coag tube.
If the patient has a known hematocrit >55%, please contact the coagulation lab for an adjusted collection tube before drawing the blood.
Interpretive Data
| Assay | Interpretive Data |
|---|---|
| APC | Activated Protein C (APC) Resistance is the screening test for Factor V Leiden. If the test is negative then the DNA assay for Factor V Leiden is not indicated and will not be performed as part of the testing panel. |
CPT(s)
85307
Performing Lab Section
Hematology