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Sputum, Cytology

eD-H Order Code


Specimen Collection Information

Specimen Container

Specimen Transport Temperature

Deliver to Lab Specimen Receiving (LSR), Borwell level 4. Refrigerate if not delivered immediately.

Specimen Stability


Additional Testing Information

Specimen Collection:
1. Rinse mouth first with warm water. A deep cough, preferably early am (first cough upon awakening). Upper tract secretions – saliva – is not acceptable.
2. A series of three early am specimens is recommended for better yield.
NOTE: If the patient cannot produce a good, deep cough specimen, the patient may be referred to inhalation therapy for sputum induction.
3. Post bronchoscopy sputum should be collected for 2 hours.
4. Complete Cytopathology Non-Gynecological requisition/order filling in all appropriate sections, indicating specimen site, laterality and including pertinent clinical data.
5. Refrigerate if there is a delay in sending specimen to the Laboratory. Send order/requisition with specimen to Lab Specimen Receiving (LSR), Borwell 4th floor.

Container: Place sputum in a prepared plastic sputum cup with cytology CytoLyt fixative. Call Central Stores/Distribution 630-650-6101 (5-6101) for supplies and delivery.
NOTE: The contents of the sputum cup should not be ingested by patient.

Label: Patient full name and date of birth or MRN (if available), as well as specimen type.

Requisition/Order: Cytopathology Non-Gynecological (form F-312) e-DH number LAB13

Required pertinent information including: patient demographics; clinical history; complete specimen identification (type, source and laterality); collection date; tests or studies requested; providers name, location and number is required. Indicate if there is a known, suspected lesion or previous malignancy or any chemotherapy/radiation or surgical therapy.

Day(s) Performed

Monday through Friday; 7:30 a.m. – 5 p.m.

Turnaround Time

48 hours upon receipt of specimen in the department, excluding holidays and weekends. If ancillary testing is required, it may require more time.

Performing Lab Section



88112, corresponding professional fees will also apply