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Brushings, Cytology

Important Note

Note: If FISH testing is requested for bile duct brushings, please send a separate collection with a miscellaneous lab request (LAB3715) order.

Additional Codes

Order Panel: Cytology Non-GYN

eD-H Order Code

LAB1912 or Cytology Non-GYN with optional Immunophenotyping Flow Cytometry (Order Panel)

Specimen Collection Information

Specimen Container
Brush in CytoLyt 

Specimen Transport Temperature

Deliver to Lab Specimen Receiving (LSR), Borwell level 4. Refrigerate if not delivered immediately.

Specimen Stability

Cytolyt solution: store the containers at 15o-30oC (59o -86oF) without cells.  Cells in Cytolyt solution are preserved for 5 days at room temperature. The 5 days at room temperature time pertains to samples in minimum Cytolyt solution to sample ratio of 1 part Cytolyt solution to 3 parts sample.  

For best results, transport specimen to laboratory immediately for processing. If there is a delay, refrigerate solution until it is delivered to the laboratory.   When transporting solution vials containing cells to the laboratory, make sure the vial is tightly sealed. Align the mark on the cap with the mark on the vial to prevent leakage.

Additional Testing Information

Brushings, Cytology Non-Gyn (Bronchial, Esophageal, Gastric, Colonic, Bile Duct, Urinary Tract, Pancreatic Duct, etc.)

NOTE: If FISH testing is requested for bile duct brushing, a separate collection and miscellaneous lab request order is needed.

Specimen Collection:

  1. Label ThinPrep CytoLyt container with patient full name and birthdate or MRN. Indicate specimen source brushed, with the laterality (and/or lobe if sampled).
  2.  Brush tip (cut off if necessary) placed directly in CytoLyt solution only – brushes received in Formalin or 95% alcohol cannot be processed. Ensure cap is tightly sealed when transporting.
  3.  Complete Cytopathology Non-Gynecological requisition/order filling in all appropriate sections, indicating specimen site, laterality and include pertinent clinical data.
  4.  Send order/requisition with specimen to Lab Specimen Receiving at DHMC, Borwell 4th floor.

Container: ThinPrep CytoLyt solution container. Call Anatomic Pathology Client Services (603-650-7211) or Central Stores/Distribution 630-650-6101 (5-6101) for supplies and delivery.

Label: Patient full name and date of birth or MRN (if available) as well as specimen type and laterality (and/or lobe if bronchial brushing).

Requisition/Order: Cytopathology Non-Gynecological (form F-312) e-DH number LAB1912

Required pertinent information including: patient demographics; clinical history; complete specimen identification (type, source and laterality); collection date; tests or studies requested; providers name, location and number is required. Indicate if there is a known, suspected lesion or previous malignancy or any chemotherapy/radiation or surgical therapy.

Day(s) Performed

Monday through Friday; 7:30 a.m. – 5 p.m.

CPT(s)

88112, corresponding professional fees will also apply

Performing Lab Section

Cytology

Turnaround Time

48 hours upon receipt of specimen in the department, excluding holidays and weekends. If ancillary testing is required, it may require more time.