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Calcitonin, Fine-Needle Aspiration Biopsy Needle Wash, Lymph Node

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Specimen Type

Fine Needle Wash


Shipping Instructions


Send specimen frozen.



Necessary Information


The biopsied site of each specimen is required and must be clearly identified in LIS and/or batch sheet.



Specimen Required


Patient Preparation: For 12 hours before this procedure do not take multivitamins or dietary supplements containing biotin (vitamin B7), which is commonly found in hair, skin, and nail supplements and multivitamins.

Collection Container/Tube: Plain, plastic, screw-top tube

Specimen Volume: 1 to 1.5mL

Collection Instructions:

1. Needle wash specimens for analysis should be collected in conjunction with cytology specimens.

2. Have saline available prior to start of procedure. Saline is the only acceptable solution for needle washings.

3. After each fine-needle aspiration biopsy (FNAB) has been collected and the material in the needle has been expelled onto a slide for cytologic analysis, attach the used FNAB needle to an empty syringe.

4. Withdraw between 0.10 mL and 0.25 mL of saline up through the needle until the saline starts to fill the hub of the needle or end of the syringe.

5. Expel this fluid back through the needle into a separate tube. This is the needle washing used for analysis.

6. Repeat steps 2 through 4 for each needle pass of the same biopsied site and empty into the same tube, accumulating a total of 0.5 mL to 1.5 mL of fluid to send to the laboratory. (If more than 1 site is biopsied, see Additional Information)

7. Inspect specimen for visible blood or tissue contamination:

-a. If bloody, centrifuge specimen and transfer supernatant to a new plastic aliquot tube (5-mL standard tube) to send to laboratory. The supernatant, not the cellular material, is used for analysis.

-b. If specimen is clear, centrifugation is not necessary.

8. Refrigerate within 1 to 2 hours of collection and freeze within 2 to 4 hours of collection.

Additional Information:

1. If more than 1 site is biopsied, each washing material should be submitted on a separate tube and under a different order number.

2. A minimum of 0.5 mL is required for testing; however, the total collection volume should not exceed 1.5 mL. Sample volumes outside these parameters may be rejected.

3. Do not send saline control. This test has been validated to rule-out saline matrix effect.


Specimen Minimum Volume

0.5 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Fine Needle Wash Frozen (preferred) 7 days
  Refrigerated  4 hours

Reference Values

An interpretive report will be provided.

Day(s) and Time(s) Performed

Monday through Friday; 6 a.m.-9 p.m.

Saturday; 8 a.m.-3 p.m.

CPT Code Information

82308

LOINC Code Information

Test ID Test Order Name Order LOINC Value
CATLN Calcitonin, FNAB, Lymph Node 75709-6

 

Result ID Test Result Name Result LOINC Value
CATL Calcitonin, FNAB, Lymph Node 75709-6
SITEB Site 39111-0

Analytic Time

Same day/1 day

Specimen Retention Time

12 months

Forms

If not ordering electronically, complete, print, and send an Oncology Test Request (T729) with the specimen.