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HelpctDNASeq panel (circulating tumor) / Liquid biopsy Assay
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DH CTDNASEQ LAB2312
Additional Testing Information
The DH Laboratory for Clinical Genomics and Advanced Technology (CGAT) now provides next generation sequencing (NGS) for detection of circulating tumor DNA from plasma using the DH-ctDNASeq assay. Effective immediately, the ctDNASeq assay will be available for patients with solid tumors. The limit of detection is 0.5% variant allele fraction (VAF).
CGAT – DHHemeSeq Gene list v1.0 (5/1/2025)
The following genes are tested for single nucleotide variants, small insertions, and deletions (46):
AKT1, ALK, APC, AR, ARID1A, ATM, BAP1, BRAF, BRCA1, BRCA2, BTK, CCND1, CDH1, CDK12, CDK4, CDK6, CDKN2A, CHEK2, CTNNB1, EGFR, ERBB2, ESR1, EZH2, FGFR1, FGFR2, FGFR3, FOXL2, GNA11, GNAQ, GNAS, HRAS, IDH1, IDH2, KIT, KRAS, MAP2K1, MAP2K2, MET, MTOR, MYC, MYCN, NF1, NOTCH1, NOTCH2, NRAS, NRG1, PDGFRA, PIK3CA, PIK3R1, PTCH1, PTEN, PTPN11, RB1, RET, ROS1, SMAD4, SMARCB1, SMO, STK11, TERT, TP53, TSC1, TSC2, VHL
Specimen Collection Information
Two 10 mL K2EDTA tubes should be drawn.
Due to specimen stability, blood samples must be drawn at 3K at Dartmouth Hitchcock Medical Center, Lebanon.
Specimen Transport
Keep at 4°C until the lab retrieves the sample.
Specimen Stability
Plasma needs to be separated in the 4 hours after collection. If a sample was not stored at 4°C or stored at 4°C for more than 48 hrs., it should be discarded.
Day(s) Performed
Monday through Friday;
TAT 10 days
Performing Laboratory
Clinical Genomics