FLT3 Mutation Analysis, Varies
Additional Codes
LAB2416
Special Instructions
Specimen Type
VariesOrdering Guidance
This test is intended to be used as a prognostic test at diagnosis and should not be used to monitor residual disease following treatment.
Shipping Instructions
Specimen must arrive within 7 days of collection.
Necessary Information
The following information is required:
1. Pertinent clinical history
2. Clinical or morphologic suspicion
3. Date and time of collection
4. Specimen source
Specimen Required
Submit only 1 of the following specimens:
Specimen Type: Whole blood
Container/Tube: Lavender top (EDTA) or yellow top (ACD)
Specimen Volume: 3 mL
Collections Instructions:
1. Invert several times to mix blood.
2. Send specimen in original tube. Do not aliquot.
3. Label specimen as whole blood.
Specimen Stability Information: Ambient (preferred) 7 days/Refrigerate 7 days
Specimen Type: Bone marrow
Container/Tube: Lavender top (EDTA) or yellow top (ACD)
Specimen Volume: 2 mL
Collections Instructions:
1. Invert several times to mix bone marrow.
2. Send specimens in original tube. Do not aliquot.
3. Label specimen as bone marrow.
Specimen Stability Information: Ambient (preferred) 7 days/Refrigerate 7 days
Specimen Type: Extracted DNA from whole blood or bone marrow
Container/Tube: 1.5- to 2-mL tube
Specimen Volume: Entire specimen
Collection Instructions:
1. DNA must be extracted from blood or bone marrow within 7 days of collection
2. Label specimen as extracted DNA and source of specimen
2. Provide volume and concentration of DNA on the label.
Specimen Stability Information: Frozen (preferred)/Refrigerate/Ambient
Additional Information: DNA must be extracted in a CLIA-certified laboratory or equivalent and must be extracted from a specimen type listed as acceptable for this test (including applicable anticoagulants). We cannot guarantee that all extraction methods are compatible with this test. If testing fails, one repeat will be attempted, and if unsuccessful, the test will be reported as failed and a charge will be applied.
Specimen Minimum Volume
Whole blood, Bone marrow: 1 mL; Extracted DNA: 50 mcL at 20 ng/mcL concentration
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Varies | Varies | 7 days |
Reference Values
An interpretive report will be provided.
Day(s) Performed
Monday through Saturday
Report Available
3 to 6 daysSpecimen Retention Time
Whole blood/Bone marrow: 2 weeks; Extracted DNA: 3 monthsPerforming Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
81245-FLT3 (fms-related tyrosine kinase) (eg, acute myeloid leukemia), gene analysis, internal tandem duplication (ITD) variants (ie, exons 14, 15)
81246-FLT3 (fms-related tyrosine kinase 3) (eg, acute myeloid leukemia), gene analysis; tyrosine kinase domain (TKD) variants (eg, D835, I836)
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| FLT | FLT3 Mutation Analysis, V | 79210-1 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| MP009 | Specimen Type | 31208-2 |
| 41935 | FLT3 Result | 79210-1 |
| 19236 | Final Diagnosis: | 34574-4 |
Forms
1. Hematopathology Patient Information (T676)
2. If not ordering electronically, complete, print, and send a Hematopathology/Cytogenetics Test Request (T726) with the specimen.