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B-Cell Phenotyping Profile for Immunodeficiency and Immune Competence Assessment, Blood

Additional Codes

LAB2017

Profile Information

Test ID Reporting Name Available Separately Always Performed
TBBS QN Lymphocyte Subsets: T, B, and NK Yes Yes
IABC Immune Assessment B Cell Subsets, B No Yes

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Specimen Type

Whole Blood EDTA


Ordering Guidance


The IABCS is a panel test. This test requires two separate whole blood EDTA specimens at two different transport temperatures: one ambient and one refrigerate.

 

The TBBS / Quantitative Lymphocyte Subsets: T, B, and Natural Killer (NK) Cells, Blood is automatically performed, a separate order is not required.

 

If only an ambient EDTA specimen is received, only the TBBS / Quantitative Lymphocyte Subsets: T, B, and Natural Killer (NK) Cells, Blood will be performed. If only a refrigerate EDTA sample is received, this test will be canceled and converted to RBCS / Relative B-Cell Subset Analysis Percentage, Blood, which provides the relative B-cell subset values without quantitation.

 

This test is a screening test and further analyses will be required to complete a diagnostic workup for hyper-IgM (XHIM / X-Linked Hyper IgM Syndrome, Blood and CD40 / B-Cell CD40 Expression by Flow Cytometry, Blood).



Shipping Instructions


Testing performed Monday through-Friday. Specimens not received by 4pm (CST) on Friday may be canceled.

 

Samples arriving on the weekend and observed holidays may be canceled.

 

Collect and package specimens as close to shipping time as possible.

 

It is recommended that specimens arrive within 24 hours of collection.



Necessary Information


1. Date of collection is required.

2. Ordering healthcare professional name and phone number are required.



Specimen Required


Two separate EDTA whole blood specimens are required: 1 refrigerate and 1 ambient transport temperature.

 

For serial monitoring, it is recommended that specimens are collected at the same time of day.

 

Specimen Type: Whole blood for TBBS / Quantitative Lymphocyte Subsets: T, B, and Natural Killer (NK) Cells, Blood

Container/Tube: 4 mL Lavender top (EDTA)

Specimen Volume: 3 mL

Collection Instructions:

1. Send whole blood specimen in original tube. Do not aliquot.

2. Label specimen as TBBS.

3. Ship ambient.

Specimen Stability Information: Ambient <52 hours

 

Specimen Type: Whole blood for IABC / B-Cell Phenotyping Screen for Immunodeficiency and Immune Competence Assessment, Blood

Container/Tube: Lavender top (EDTA)

Specimen Volume:

≤14 years: 4 mL

>14 years: 10 mL

Collection Instructions:

1. Send whole blood specimen in original tube. Do not aliquot.

2. Label specimen IABC.

3. Ship refrigerate.

Specimen Stability Information: Refrigerated <48 hours


Specimen Minimum Volume

TBBS: 1 mL; IABC: > 14 years: 5 mL; ≤ 14 years: 3 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Whole Blood EDTA Varies 48 hours PURPLE OR PINK TOP/EDTA

Reference Values

The appropriate age-related reference values will be provided on the report.

Day(s) Performed

Monday through Friday

CPT Code Information

86355-B cells, total count

86357-Natural killer (NK) cells, total count

86359-T cells, total count

86360-Absolute CD4/CD8 count with ratio

86356 x7 - Mononuclear cell antigen, quantitative

LOINC Code Information

Test ID Test Order Name Order LOINC Value
IABCS Immune Assessment B Cell Subsets, B 90416-9

 

Result ID Test Result Name Result LOINC Value
30296 CD19+ % of total Lymphocytes 8117-4
3321 CD45 Total Lymph Count 27071-0
3316 % CD3 (T Cells) 8124-0
29094 CD20+ % of total Lymphocytes 8119-0
3322 CD3 (T Cells) 8122-4
30298 CD27+ % of CD19+ B cells 89358-6
3319 % CD4 (T Cells) 8123-2
30300 CD27+ IgM+ IgD+ % of CD19+ B cells 89352-9
3325 CD4 (T Cells) 24467-3
30302 CD27+ IgM- IgD- % of CD19+ B cells 89350-3
3320 % CD8 (T Cells) 8101-8
30304 CD27+ IgM+ IgD- % of CD19+ B cells 89348-7
3326 CD8 (T Cells) 14135-8
30306 IgM+ % of CD19+ B cells 89346-1
3318 % CD19 (B Cells) 8117-4
30308 CD38+ IgM- % of CD19+ B cells 89344-6
30310 CD38+ IgM+ % of CD19+ B cells 89341-2
3324 CD19 (B Cells) 8116-6
4054 % CD16+CD56 (NK cells) 8112-5
30312 CD21+ % of CD19+ B cells 89356-0
30314 CD21- % of CD19+ B cells 89355-2
4055 CD16+CD56 (NK cells) 20402-4
3327 4/8 Ratio 54218-3
30297 CD19+ 8116-6
29095 CD20+ 9558-8
6657 Comment 80722-2
30299 CD27+ 89353-7
30301 CD27+ IgM+ IgD+ 89351-1
30303 CD27+ IgM- IgD- 89349-5
30305 CD27+ IgM+ IgD- 89347-9
30307 IgM+ 89345-3
30309 CD38+ IgM- 89343-8
30311 CD38+ IgM+ 89357-8
30313 CD21+ 25164-5
30315 CD21- 89354-5
30316 Interpretation 80722-2

Report Available

3 to 4 days

Specimen Retention Time

PBMC:7 days