B-Cell Phenotyping Profile for Immunodeficiency and Immune Competence Assessment, Blood
Additional Codes
LAB2017
Profile Information
Test ID | Reporting Name | Available Separately | Always Performed |
---|---|---|---|
TBBS | QN Lymphocyte Subsets: T, B, and NK | Yes | Yes |
IABC | Immune Assessment B Cell Subsets, B | No | Yes |
Performing Laboratory
Mayo Clinic Laboratories in RochesterSpecimen Type
Whole Blood EDTAOrdering Guidance
The IABCS is a panel test. This test requires two separate whole blood EDTA specimens at two different transport temperatures: one ambient and one refrigerate.
The TBBS / Quantitative Lymphocyte Subsets: T, B, and Natural Killer (NK) Cells, Blood is automatically performed, a separate order is not required.
If only an ambient EDTA specimen is received, only the TBBS / Quantitative Lymphocyte Subsets: T, B, and Natural Killer (NK) Cells, Blood will be performed. If only a refrigerate EDTA sample is received, this test will be canceled and converted to RBCS / Relative B-Cell Subset Analysis Percentage, Blood, which provides the relative B-cell subset values without quantitation.
This test is a screening test and further analyses will be required to complete a diagnostic workup for hyper-IgM (XHIM / X-Linked Hyper IgM Syndrome, Blood and CD40 / B-Cell CD40 Expression by Flow Cytometry, Blood).
Shipping Instructions
Testing performed Monday through-Friday. Specimens not received by 4pm (CST) on Friday may be canceled.
Samples arriving on the weekend and observed holidays may be canceled.
Collect and package specimens as close to shipping time as possible.
It is recommended that specimens arrive within 24 hours of collection.
Necessary Information
1. Date of collection is required.
2. Ordering healthcare professional name and phone number are required.
Specimen Required
Two separate EDTA whole blood specimens are required: 1 refrigerate and 1 ambient transport temperature.
For serial monitoring, it is recommended that specimens are collected at the same time of day.
Specimen Type: Whole blood for TBBS / Quantitative Lymphocyte Subsets: T, B, and Natural Killer (NK) Cells, Blood
Container/Tube: 4 mL Lavender top (EDTA)
Specimen Volume: 3 mL
Collection Instructions:
1. Send whole blood specimen in original tube. Do not aliquot.
2. Label specimen as TBBS.
3. Ship ambient.
Specimen Stability Information: Ambient <52 hours
Specimen Type: Whole blood for IABC / B-Cell Phenotyping Screen for Immunodeficiency and Immune Competence Assessment, Blood
Container/Tube: Lavender top (EDTA)
Specimen Volume:
≤14 years: 4 mL
>14 years: 10 mL
Collection Instructions:
1. Send whole blood specimen in original tube. Do not aliquot.
2. Label specimen IABC.
3. Ship refrigerate.
Specimen Stability Information: Refrigerated <48 hours
Specimen Minimum Volume
TBBS: 1 mL; IABC: > 14 years: 5 mL; ≤ 14 years: 3 mL
Specimen Stability Information
Specimen Type | Temperature | Time | Special Container |
---|---|---|---|
Whole Blood EDTA | Varies | 48 hours | PURPLE OR PINK TOP/EDTA |
Reference Values
The appropriate age-related reference values will be provided on the report.
Day(s) Performed
Monday through Friday
CPT Code Information
86355-B cells, total count
86357-Natural killer (NK) cells, total count
86359-T cells, total count
86360-Absolute CD4/CD8 count with ratio
86356 x7 - Mononuclear cell antigen, quantitative
LOINC Code Information
Test ID | Test Order Name | Order LOINC Value |
---|---|---|
IABCS | Immune Assessment B Cell Subsets, B | 90416-9 |
Result ID | Test Result Name | Result LOINC Value |
---|---|---|
30296 | CD19+ % of total Lymphocytes | 8117-4 |
3321 | CD45 Total Lymph Count | 27071-0 |
3316 | % CD3 (T Cells) | 8124-0 |
29094 | CD20+ % of total Lymphocytes | 8119-0 |
3322 | CD3 (T Cells) | 8122-4 |
30298 | CD27+ % of CD19+ B cells | 89358-6 |
3319 | % CD4 (T Cells) | 8123-2 |
30300 | CD27+ IgM+ IgD+ % of CD19+ B cells | 89352-9 |
3325 | CD4 (T Cells) | 24467-3 |
30302 | CD27+ IgM- IgD- % of CD19+ B cells | 89350-3 |
3320 | % CD8 (T Cells) | 8101-8 |
30304 | CD27+ IgM+ IgD- % of CD19+ B cells | 89348-7 |
3326 | CD8 (T Cells) | 14135-8 |
30306 | IgM+ % of CD19+ B cells | 89346-1 |
3318 | % CD19 (B Cells) | 8117-4 |
30308 | CD38+ IgM- % of CD19+ B cells | 89344-6 |
30310 | CD38+ IgM+ % of CD19+ B cells | 89341-2 |
3324 | CD19 (B Cells) | 8116-6 |
4054 | % CD16+CD56 (NK cells) | 8112-5 |
30312 | CD21+ % of CD19+ B cells | 89356-0 |
30314 | CD21- % of CD19+ B cells | 89355-2 |
4055 | CD16+CD56 (NK cells) | 20402-4 |
3327 | 4/8 Ratio | 54218-3 |
30297 | CD19+ | 8116-6 |
29095 | CD20+ | 9558-8 |
6657 | Comment | 80722-2 |
30299 | CD27+ | 89353-7 |
30301 | CD27+ IgM+ IgD+ | 89351-1 |
30303 | CD27+ IgM- IgD- | 89349-5 |
30305 | CD27+ IgM+ IgD- | 89347-9 |
30307 | IgM+ | 89345-3 |
30309 | CD38+ IgM- | 89343-8 |
30311 | CD38+ IgM+ | 89357-8 |
30313 | CD21+ | 25164-5 |
30315 | CD21- | 89354-5 |
30316 | Interpretation | 80722-2 |