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HelpLymphocyte Proliferation to Antigens, Blood
Additional Codes
LAB4319
WBC Antigens, Proliferation
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Specimen Type
WB Sodium HeparinOrdering Guidance
This test should not be ordered for patients younger than 3 months unless there is a clinical history of candidiasis. For more information see Cautions.
Shipping Instructions
Testing performed Monday through Friday. Specimens not received by 4 p.m. Central time on Friday may be canceled.
Specimens arriving on the weekend and observed holidays may be canceled.
Collect and package specimen as close to shipping time as possible. Ship specimen overnight in an Ambient Shipping Box-Critical Specimens Only (T668) following the instructions in the box. It is recommended that specimens arrive within 24 hours of collection.
Necessary Information
1. Date and time of collection
2. The ordering healthcare professional's name and phone number are required.
Specimen Required
Supplies: Ambient Shipping Box-Critical Specimens Only (T668)
Container/Tube: Green top (sodium heparin)
Specimen Volume: 20 mL
See tables for information on recommended volume based on absolute lymphocyte count
Pediatric Volume:
<3 months: 1 mL
3-24 months: 3 mL
25 months-18 years: 5 mL
Collection Instructions: Send whole blood specimen in original tube. Do not aliquot.
Additional Information: For serial monitoring, it is recommended that specimen collection be performed at the same time of day.
Table. Blood Volume Recommendations Based on Absolute Lymphocyte Count (ALC)
|
Antigen only |
||
|
ALC x 10(9)/L |
Blood volume for minimum Candida albicans (CA) and tetanus toxoid (TT) Only |
Blood volume for full assay |
|
<0.5 |
>18.5 mL |
>40 mL |
|
0.5-1.0 |
18.5 mL |
40 mL |
|
1.1-1.5 |
8.5 mL |
20 mL |
|
1.6-2.0 |
6.0 mL |
12 mL |
|
2.1-3.0 |
4.5 mL |
10 mL |
|
3.1-4.0 |
3.0 mL |
6 mL |
|
4.1-5.0 |
2.5 mL |
5 mL |
|
>5.0 |
2.0 mL |
4 mL |
|
Mitogen and antigen |
||
|
ALC x 10(9)/L |
Blood volume for minimum of each assay |
Blood volume for full assay |
|
<0.5 |
>28 mL |
>60 mL |
|
0.5-1.0 |
28 mL |
60 mL |
|
1.1-1.5 |
12 mL |
30 mL |
|
1.6-2.0 |
8.5 mL |
20 mL |
|
2.1-3.0 |
6.5 mL |
15 mL |
|
3.1-4.0 |
4.5 mL |
10 mL |
|
4.1-5.0 |
3.5 mL |
8 mL |
|
>5.0 |
2.5 mL |
6 mL |
Specimen Minimum Volume
<6 years: 1 mL; 6-18 years: 2 mL; >18 years: 6 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| WB Sodium Heparin | Ambient | 48 hours | GREEN TOP/HEP |
Reference Values
Viability of lymphocytes at day 0: ≥75.0%
Maximum proliferation of Candida albicans as % CD45: ≥5.7%
Maximum proliferation of Candida albicans as % CD3: ≥3.0%
Maximum proliferation of tetanus toxoid as % CD45: ≥5.2%
Maximum proliferation of tetanus toxoid as % CD3: ≥3.3%
Day(s) Performed
Monday through Friday
CPT Code Information
86353
86353 (if appropriate)
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| LPAGF | Lymphocyte Proliferation, Antigens | 69042-0 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 32325 | Interpretation | 69052-9 |
| 32326 | Viab of Lymphs at Day 0 | 33193-4 |
| 32327 | Max Prolif of CA as % CD45 | 69014-9 |
| 32328 | Max Prolif of CA as % CD3 | 69015-6 |
| 32329 | Max Prolif of TT as % CD45 | 69016-4 |
| 32330 | Max Prolif of TT as % CD3 | 69029-7 |
| 32331 | Antigen Comment | 48767-8 |
Report Available
11 to 14 daysSpecimen Retention Time
Not retained. Entire specimen is used in preparation of the assayReflex Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| AGSTM | Additional Flow Stimulant, LPAGF | No, (Bill Only) | No |