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Lyme Disease, Molecular Detection, PCR, Varies

Additional Codes

Order in eDH using Miscellaneous Lab Request (LAB3715).

This test requires Pathologist approval and can only be ordered in Cerner Millennium by Mailouts Dept (Lyme PCR-Mayo).

 


Ordering Guidance


This assay does not detect Borrelia miyamotoi. If infection with this organism is suspected, order BMIPB / Borrelia miyamotoi Detection, PCR, Blood or BMIYC / Borrelia miyamotoi Detection, PCR, Spinal Fluid.



Necessary Information


Specimen source is required.



Specimen Required


Submit only 1 of the following specimens:

 

Specimen Type: Spinal fluid

Container/Tube: Sterile vial

Specimen Volume: 1 mL

Collection Instructions: Label specimen as spinal fluid.

 

Specimen Type: Synovial fluid

Container/Tube: Sterile vial

Specimen Volume: 1 mL

Collection Instructions: Label specimen as synovial fluid.

 

Specimen Type: Tissue (fresh only)

Sources: Skin or synovial biopsy

Container/Tube: Sterile container with normal saline

Specimen Volume: Approximately 4 mm(3)

Collection Instructions:

1. Submit only fresh tissue.

2. Skin biopsies:

a. Wash biopsy site with an antiseptic soap. Thoroughly rinse area with sterile water. Do not use alcohol or iodine preparations. A local anesthetic may be used.

b. Biopsy specimens are best taken by punch biopsy to include full thickness of dermis.

3. Label specimen with source of tissue.


Specimen Type

Varies

Specimen Minimum Volume

Spinal Fluid: 0.3 mL; Synovial Fluid: 0.5 mL; Tissue: See Specimen Required

Specimen Stability Information

Specimen Type Temperature Time Special Container
Varies Refrigerated (preferred) 7 days
  Frozen  7 days

Reference Values

Negative

Reference values apply to all ages.

Day(s) Performed

June through November: Monday through Saturday

December through May: Monday through Friday

Report Available

Same day/ 1 to 4 days

Specimen Retention Time

1 week

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

87476

87798 x 2

87999 (if appropriate for government payers)

LOINC Code Information

Test ID Test Order Name Order LOINC Value
LYMPV Lyme Disease, PCR, Varies 94253-2

 

Result ID Test Result Name Result LOINC Value
LYMS Specimen Source 31208-2
618333 B. burgdorferi PCR 94250-8
618334 B. mayonii PCR 94251-6
618335 B. garinii/B. afzelii PCR 94252-4
618336 Lyme CSF Comment 59464-8

Forms

If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.