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Mycobacterium tuberculosis Complex, Molecular Detection, PCR, Paraffin, Tissue

Specimen Type

Tissue, Paraffin


Additional Testing Requirements


When non-fixed specimen is available, a mycobacterial culture on that specimen must always be performed in addition to this test. If your facility is unable to perform mycobacterial culture, order CTB / Mycobacteria and Nocardia Culture, Varies concurrently with this test.



Necessary Information


Specimen source is required.



Specimen Required


The high sensitivity of amplification by polymerase chain reaction requires the specimen to be processed in an environment in which contamination of the specimen by Mycobacterium tuberculosis DNA is unlikely.

 

Preferred:

Supplies: Tissue Block Container (T553)

Specimen Type: Formalin-fixed, paraffin-embedded (FFPE) tissue block

Sources: Body tissue

Container/Tube: Tissue block

Collection Instructions: Submit a FFPE tissue block to be cut and returned.

 

Acceptable:

Specimen Type: Formalin-fixed, paraffin-embedded (FFPE) tissue sections (scrolls)

Sources: Body tissue

Container/Tube: Sterile containers, one for each individual cut section (scroll).

Collection Instructions:

1. Perform microtomy and prepare five separate 10-micron sections.

2. Place each section (scroll) in a separate sterile container for submission.


Specimen Minimum Volume

See Specimen Required.

Specimen Stability Information

Specimen Type Temperature Time
Tissue, Paraffin Ambient (preferred)
  Refrigerated 

Reference Values

Not applicable

Day(s) Performed

Monday through Sunday

Report Available

5 to 7 days

Specimen Retention Time

7 days; after which time the block will be returned to the client

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

87556

LOINC Code Information

Test ID Test Order Name Order LOINC Value
MTBT MTB complex PCR, FFPE 38379-4

 

Result ID Test Result Name Result LOINC Value
SRCTB MTB Complex PCR, FFPE, Source 31208-2
TBRR MTB Complex PCR, FFPE, Result 38379-4