Hematologic Neoplasms, TP53 Somatic Mutation, DNA Sequencing Exons 4-9, Varies
Specimen Type
VariesOrdering Guidance
For the evaluation of patients suspected of having an inherited or germline TP53 cancer syndrome (eg, Li Fraumeni syndrome), order one of the following tests containing TP53:
-XCP/ Hereditary Expanded Cancer Panel, Varies
-COMCP / Hereditary Common Cancer Panel, Varies
-BRGYP / Hereditary Breast/Gynecologic Cancer Panel, Varies
-CRCGP / Hereditary Gastrointestinal Cancer Panel, Varies
-PANCP / Hereditary Pancreatic Cancer Panel, Varies
-ENDCP / Hereditary Endocrine Cancer Panel, Varies
-THYRP / Hereditary Thyroid Cancer Panel, Varies
-WILMP / Hereditary Wilms Tumor Panel, Varies
-RENCP / Hereditary Renal Cancer Panel, Varies
-PRS8P / Hereditary Prostate Cancer Panel, Varies
Shipping Instructions
Blood and bone marrow specimens must arrive within 10 days of collection.
Necessary Information
The following information is required:
1. Pertinent clinical history
2. Clinical or morphologic suspicion
3. Date of collection
4. Specimen source
Specimen Required
Submit only 1 of the following specimens:
Specimen Type: Blood (preferred)
Container/Tube: Lavender top (EDTA) or yellow top (ACD solution B)
Specimen Volume: 3 mL
Collection Instructions:
1. Invert several times to mix blood.
2. Send whole blood specimen in original tube. Do not aliquot.
3. Label specimen as blood.
Specimen Stability Information: Ambient/Refrigerate <10 days
Specimen Type: Bone marrow
Container/Tube: Lavender top (EDTA), yellow top (ACD solution B), or green top (heparin)
Specimen Volume: 3 mL
Collection Instructions:
1. Invert several times to mix bone marrow.
2. Send bone marrow specimen in original tube. Do not aliquot.
3. Label specimen as bone marrow.
Specimen Stability Information: Ambient/Refrigerate <10 days
Specimen Type: Tissue
Container/Tube: Plastic container
Specimen Volume: 100 mg
Collection Instructions: Stabilize fresh tissue in tissue culture medium or freeze immediately after collection.
Specimen Stability Information: Refrigerate 24 hours/ Frozen
Specimen Minimum Volume
Blood, bone marrow: 1 mL
Specimen Stability Information
Specimen Type | Temperature | Time | Special Container |
---|---|---|---|
Varies | Varies | 10 days |
Reference Values
Genetic variants present or absent as compared to a reference sequence of the normal TP53 gene
Day(s) Performed
Monday through Friday
Report Available
8 to 14 daysSpecimen Retention Time
Blood/Bone marrow: 2 weeks; Extracted DNA 3 monthsPerforming Laboratory
Mayo Clinic Laboratories in RochesterCPT Code Information
81352-TP53 (tumor protein 53) (eg, tumor samples), full gene sequence or targeted sequence analysis of >5 exons
LOINC Code Information
Test ID | Test Order Name | Order LOINC Value |
---|---|---|
P53CA | TP53 gene somatic mutation analysis | 21739-8 |
Result ID | Test Result Name | Result LOINC Value |
---|---|---|
MP018 | Specimen Type: | 31208-2 |
607075 | Signing Pathologist | 19139-5 |
35759 | Final Diagnosis: | 34574-4 |
Reflex Tests
Test ID | Reporting Name | Available Separately | Always Performed |
---|---|---|---|
CSP53 | TP53 Pre-Analysis Cell Sorting, V | No | No |
Special Instructions
Forms
2. If not ordering electronically, complete, print, and send a Hematopathology/Cytogenetics Test Request (T726) with the specimen.